|
KMID : 1123920100240020296
|
|
Korean Journal of Oriental Physiology and Pathology
2010 Volume.24 No. 2 p.296 ~ p.301
|
|
|
Inhibitory Effect of Fructus Ligustri Lucidi on Tyrosinase and MITF Expressions
|
|
Han Kyu-Soo
Kim Dae-Sung
Woo Won-Hong
Mun Yeun-Ja
|
|
|
Abstract
|
|
|
|
The purpose of this study was to investigate the mechanism of Hexane extract of Fructus Ligustri Lucidi (HFLL)-induced regulation of melanogenesis. An apparent down-regulatory effect of tyrosinase activity was observed when B16F10 cells were incubated with HFLL. Interestingly, HFLL did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 cells, whereas kojic acid directly inhibited tyrosinase activity. Regarding protein levels of melanogenic enzymes, the amounts of tyrosinase and tyrosinase-related protein 1 (TRP-1) were decreased by HFLL, while the amount of tyrosinase-related protein 2 (TRP-2) slightly was reduced after incubation with HFLL. Treatment with HFLL was found to down-regulate microphthalmia-associated transcription factor (MITF). These results suggest that HFLL is an effective inhibitor of pigmentation caused by down regulation via MITF, tyrosinase, and TRP-1 expressions.
|
|
|
KEYWORD
|
|
|
Fructus Ligustri Lucidi, melanin, tyrosinase, depigmentation, MITF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|